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KMID : 1094720140190050908
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Biotechnology and Bioprocess Engineering
2014 Volume.19 No. 5 p.908 ~ p.915
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Enhancement of taxol production from endophytic fungus Fusarium redolens
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Garyali Sanjog
Kumar Anil
Reddy M. Sudhakara
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Abstract
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The optimization of taxol production by Fusarium redolens by one factor at a time (OFAT) approach led to production of 70 μg/L of taxol. With sucrose and NH4NO3 as the carbon and nitrogen sources and medium volume (V m ) to flask volume (V f ) ratio of 0.2, a greater taxol production was attained. NH4NO3, MgSO4↔7H2O and NaOAc at 6.25, 0.63, and 1.25 g/L, were the significant factors for attaining the highest taxol production. The optimization of culture variables led to the production of taxol from 66 to 198 μg/L, which is three fold higher than that in the unoptimized medium. Current study results suggested the success of Response Surface Methodology in enhancing the production of fungal taxol.
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KEYWORD
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taxol, Fusarium redolens, Plackett-Burman design, response surface methodology
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